Reporter gene fusions.

نویسندگان

  • Thomas Boulin
  • John F Etchberger
  • Oliver Hobert
چکیده

Copyright: © 2006 Thomas Boulin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Both of these authors contributed equally To whom correspondence should be addressed. E-mail: [email protected] Reporter gene fusions* Thomas Boulin, John F. Etchberger, Oliver Hobert, Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Center for Neurobiology and Behavior, Columbia University, New York, NY 10032 USA

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Rapid engineering of bacterial reporter gene fusions by using Red recombination.

Reporter gene fusions are essential tools for the investigation of gene regulation. Such fusions are traditionally generated by transposon mutagenesis and identified by a suitable selection procedure. Alternatively, specific reporter fusions can be generated by cloning of DNA fragments containing promoters or other regulatory elements in reporter plasmids. Here, we describe a novel approach for...

متن کامل

A New Reporter Gene Technology: Opportunities and Perspectives

The paper summarizes the current status of the reporter gene technology and their basics. Reporter gene technology is widely used to monitor cellular events associated with gene expression and signal transduction. Based upon the splicing of transcriptional control elements to a variety of reporter genes, it “reports” the effects of a cascade of signaling events on gene expression inside cells. ...

متن کامل

LuxArray, a high-density, genomewide transcription analysis of Escherichia coli using bioluminescent reporter strains.

A sequenced collection of plasmid-borne random fusions of Escherichia coli DNA to a Photorhabdus luminescens luxCDABE reporter was used as a starting point to select a set of 689 nonredundant functional gene fusions. This group, called LuxArray 1.0, represented 27% of the predicted transcriptional units in E. coli. High-density printing of the LuxArray 1.0 reporter strains to membranes on agar ...

متن کامل

Assaying Chimeric Genes in Plants: The GUS Gene Fusion System

DeJi~eitio, r Ge~e lrlt.~irm Much of tile attention and interest in modern molecular biology is fi~cussed on the regulation of gene expression. Factors influencing or mediating such regulation are often best studied using gene Alsions. Gene fusions can be defined its DNA constructions (perfi3rmed ill vitro or i~e Hvo) that result in the coding sequences from one gene (r@o,ter) being transcribed...

متن کامل

Expression analysis of the yersiniabactin receptor gene fyuA and the heme receptor hemR of Yersinia enterocolitica in vitro and in vivo using the reporter genes for green fluorescent protein and luciferase.

The enteropathogenic Yersinia enterocolitica strains have several systems for scavenging iron from their environment. We have studied the expression of the fyuA gene, which encodes the outer membrane receptor for the siderophore yersiniabactin (Ybt), and the hemR gene, which encodes the receptor for heme, using the reporter genes gfp (encoding green fluorescent protein) and luc (encoding firefl...

متن کامل

An Alkaline Phosphatase Reporter Gene Assay for Induction of CYP3A4 In Vitro

CYP3A4 probably has the broadest catalytic activity of any cytochrome P450. It is a crucial task to test new drug candidates in a reliable system for their ability to induce expression of this enzyme. Firstly, a total of 300 bp core distal enhancer of CYP3A4 XREM region (-7972/-7673) were amplified from human genomic DNA. The PCR product was then ligated into a human secretory alkaline phosphat...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • WormBook : the online review of C. elegans biology

دوره   شماره 

صفحات  -

تاریخ انتشار 2006